cdna synthesis (New England Biolabs)
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New England Biolabs
cdna synthesis
Cdna Synthesis, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protoscript+ii+reverse+transcriptase/ProtoScript+II+Reverse+Transcriptase/pmc13178204-68-20-24
Average 98 stars, based on 1438 article reviews
Cdna Synthesis, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protoscript+ii+reverse+transcriptase/ProtoScript+II+Reverse+Transcriptase/pmc13178204-68-20-24
Average 98 stars, based on 1438 article reviews
cdna synthesis - by Bioz Stars,
2026-09
98/100 stars
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Reverse Transcription:Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells. Article Snippet: The RNA of the RNA-DNA was fragmented in buffer (4 μL of 5× Protoscript II buffer, M0368S, NEB; 1 μL of 10 mM dNTPs) with a primer complementary to the adaptor oligo (5’CCGCTTATCGAACTGCTG, 20 pmol per reaction) at 94 °C for 6 min prior to reverse transcription. .. The RNA-DNA was reverse transcribed using Article Title: Linking oxidative stress defense to biofilm architecture: Ohr mediates strain-dependent persistence in Acinetobacter baumannii Article Snippet: Ribosomal RNA (rRNA) was depleted from total RNA using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, San Diego, CA, USA). .. First-strand cDNA was synthesized with Article Title: Host GPCR-cAMP signaling balances Gαs and Gαi activity to control intracellular Brucella infection. Article Snippet: Total RNA was extracted using the Direct-zol RNA Miniprep Kit (Zymo Research) with on-column DNase treatment. .. One microgram of RNA was reverse-transcribed using Article Title: Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19 Article Snippet: Total RNA was purified with the RNeasy Plus kit (74134, Qiagen) or Direct-zol DNA/RNA Miniprep (R2080, Zymo Research). .. Reverse transcription was performed using Article Title: Regulation of YAP activity by nuclear G-actin binding Article Snippet: .. RNA and primer were denatured in a 10 μL reaction with 1 μg total RNA, 2 μL Oligo d(T)23 VN (New England Biolabs, S1327S), 1 μL 10 mM dNTP (New England Biolabs, N0447S) for 5 min at 65°C, then cDNA synthesis was synthesized at 42°C for 1 h after adding 2.8 μL Nuclease-free H 2 O (New England Biolabs, B1500L), 4 μL 5X ProtoScript II Buffer, 2 μL 0.1M DTT (Roth, 6908.1), 0.2 μL RNase Inhibitor (40 U/μl) (New England Biolabs, M0307S) and 1 μL Article Title: The conserved nematode pheromone ascr#18 primes plant immunity. Article Snippet: DNA contamination was removed by treatment with DNase I (Invitrogen, USA). .. First-strand cDNA was synthetized from 50 ng of mRNA using Article Title: Unmasking the common enemy: drug resistance mechanisms across three different EGFR inhibitor generations are associated with co-targetable alterations in extracellular matrix signaling. Article Snippet: Droplet digital PCR (ddPCR) oils and ddPCR supermix for probes (no dUTP) were obtained from Bio-Rad Laboratories (Hercules, CA, USA). .. Q5® Hot Start High-Fidelity DNA Polymerase (supplied with 5X Q5® Reaction Buffer and 5X Q5® High GC Enhancer), Article Title: Proximity proteomics reveals a role for IFI16 during human coronavirus infection Article Snippet: Total RNA was harvested and quantified using a NanoDrop (Thermo Scientific). .. A total of 300 ng of RNA was used to generate cDNA using Synthesized:Article Title: Linking oxidative stress defense to biofilm architecture: Ohr mediates strain-dependent persistence in Acinetobacter baumannii Article Snippet: Ribosomal RNA (rRNA) was depleted from total RNA using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, San Diego, CA, USA). .. First-strand cDNA was synthesized with Article Title: Regulation of YAP activity by nuclear G-actin binding Article Snippet: .. RNA and primer were denatured in a 10 μL reaction with 1 μg total RNA, 2 μL Oligo d(T)23 VN (New England Biolabs, S1327S), 1 μL 10 mM dNTP (New England Biolabs, N0447S) for 5 min at 65°C, then cDNA synthesis was synthesized at 42°C for 1 h after adding 2.8 μL Nuclease-free H 2 O (New England Biolabs, B1500L), 4 μL 5X ProtoScript II Buffer, 2 μL 0.1M DTT (Roth, 6908.1), 0.2 μL RNase Inhibitor (40 U/μl) (New England Biolabs, M0307S) and 1 μL Amplification:Article Title: Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19 Article Snippet: Total RNA was purified with the RNeasy Plus kit (74134, Qiagen) or Direct-zol DNA/RNA Miniprep (R2080, Zymo Research). .. Reverse transcription was performed using Real-time Polymerase Chain Reaction:Article Title: Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19 Article Snippet: Total RNA was purified with the RNeasy Plus kit (74134, Qiagen) or Direct-zol DNA/RNA Miniprep (R2080, Zymo Research). .. Reverse transcription was performed using Article Title: Proximity proteomics reveals a role for IFI16 during human coronavirus infection Article Snippet: Total RNA was harvested and quantified using a NanoDrop (Thermo Scientific). .. A total of 300 ng of RNA was used to generate cDNA using cDNA Synthesis:Article Title: Regulation of YAP activity by nuclear G-actin binding Article Snippet: .. RNA and primer were denatured in a 10 μL reaction with 1 μg total RNA, 2 μL Oligo d(T)23 VN (New England Biolabs, S1327S), 1 μL 10 mM dNTP (New England Biolabs, N0447S) for 5 min at 65°C, then cDNA synthesis was synthesized at 42°C for 1 h after adding 2.8 μL Nuclease-free H 2 O (New England Biolabs, B1500L), 4 μL 5X ProtoScript II Buffer, 2 μL 0.1M DTT (Roth, 6908.1), 0.2 μL RNase Inhibitor (40 U/μl) (New England Biolabs, M0307S) and 1 μL |